Culture-independent approaches
23 important questions on Culture-independent approaches
How do culture independent methods overcome the limitation that not all microorganisms can be cultured?
What does 16S rRNA gene analysis give an overview of?
What does functional gene analysis give an overview of?
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What do culture independent methods allow?
What are disadvantages of culture independent methods?
Why is the 16S rRNA commonly used as a phylogenetic marker?
What is the function of the 16S rRNA?
What are general/universal primers?
What can general/universal primers be used for?
What are group specific primers?
What can group specific primers be used for?
What is the difference between the ssRNA variable and conserved region?
How can the product be visualized after a PCR?
What method can be used to differentiate between the cells that contain PCR product from the ones without the PCR product?
What does it mean when the colonies are white in the blue-white screening?
What does it mean when the colonies are blue in the blue-white screening?
What three components are present in the selective agar for the blue-white screening?
What is the function of the antibiotic in the selective media?
What is the function of the X-gal in the selective media?
What is the function of IPTG?
How is the blue pigment in the blue-white screening caused?
What happens if the LacZ gene is disrupted?
What is a rarefaction curve?
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