Microbial ecology and environmental microbiology - Classical procedures for Isolating Microbes

4 important questions on Microbial ecology and environmental microbiology - Classical procedures for Isolating Microbes

Common isolation procedures include the streak plate method, agar dilution and liquid dilution. What do these three methods involve?

The streak plate method involves spreading a sample onto a solid agar plate in a way that isolates individual bacterial colonies. Agar dilution involves preparing a series of agar plates with different concentrations of an antimicrobial agent to determine the minimum inhibitory concentration. Liquid dilution involves preparing a series of liquid nutrient tubes with different dilutions of a sample to isolate individual bacterial colonies. All three methods are used to separate and isolate bacterial colonies for further study, allowing researchers to identify and characterize specific microorganisms in a sample.

What is the agar dilution tube method?

The agar dilution tube method is a microbiological technique used to determine the minimum inhibitory concentration (MIC) of antimicrobial agents against bacteria or fungi. In this method, various concentrations of the antimicrobial agent are incorporated into tubes containing solid agar. The tubes are then inoculated with the microorganism of interest to observe which concentration inhibits its growth, thereby determining the MIC.

What is the liquid dilution method?

The liquid dilution method is a technique used to reduce the concentration of a particular substance in a liquid solution by adding more solvent. This is done by measuring out a specific volume of the original solution and then adding a calculated volume of solvent to reach the desired dilution factor. The resulting solution will have a lower concentration of the original substance.
  • Higher grades + faster learning
  • Never study anything twice
  • 100% sure, 100% understanding
Discover Study Smart

Once a putative culture has been obtained, it is essential to verify its purity. How do you do this? (s.653)

To verify the purity of a putative culture, you can perform streak plate method by streaking the culture on agar plate, observe for single isolated colonies. You can also perform microscopic examination to check for different cell morphologies. Additionally, biochemical tests or DNA/RNA analysis can be done to confirm the identity of the culture.

The question on the page originate from the summary of the following study material:

  • A unique study and practice tool
  • Never study anything twice again
  • Get the grades you hope for
  • 100% sure, 100% understanding
Remember faster, study better. Scientifically proven.
Trustpilot Logo