The Microbial World - Imaging Cells in Three Dimensions

3 important questions on The Microbial World - Imaging Cells in Three Dimensions

To visualize cells in three dimensions, which two different methods can be used?

  • Differential Interference Contrast microscopy
  • Confocal Scanning Laser microscopy

What is Differential Interference Contrast microscopy?

Differential interference contrast (DIC) microscopy uses a polarizer to create two distinct beams of polarized light. It gives structures such as endospores, vacuoles, and granules a three-dimensional appearance. Structures that are not visible by bright-field microscopy are sometimes visible by DIC.

What is confocal scanning laser microscopy?

Confocal scanning laser microscopy (CSLM) uses a computerized microscope coupled with a laser source to generate a three-dimensional image. A computer can focus the laser on single layers of the specimen. Different layers can then be compiled for a three-dimensional image. The resolution is 0.1 μm for CSLM.

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