Viruses and Their Replication - Replication of Bacteriophage T4

4 important questions on Viruses and Their Replication - Replication of Bacteriophage T4

What three parts can the multiplication process of T4 be divided into?

  • Early processes
  • Middle processes
  • Late processes

What happens in the early process of T4 multiplication?

In the early process of T4 multiplication the early enzymes and proteins are synthesized from the early mRNA:

  • Enzyme for the synthesis and glucosylation of the T4 base hydroxymethylcytosine
  • Enzymes that function in T4 genome replication
  • Proteins that modify host RNA polymerase

What happens in the late process of T4 multiplication?

In the late process of T4 multiplication the late proteins are made from the late mRNA. These proteins include proteins of the virus coat.
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What is the packaging process of the T4?

  1. Precursors of the bacteriophage head, called proheads, are assembled but remain empty.
  2. A packaging motor is assembled at the opening to the prohead and the double stranded linear T4 DNA genome is pumped into the prohead using ATP as the driving force.
  3. The packaging motor itself is discarded and the capsid head is sealed.

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