2016 Final, Koch & Robertson

50 important questions on 2016 Final, Koch & Robertson

Mendel's second law of independent assortment has to do with

Meiotic division in homologue chromosomes

The genetic term "incomplete dominance" refers to

Blending the parental traits creating a third phenotype

Many purebred dogs have higher prevalence than mixed-breed dogs of

All of the above
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Genomic estimated breeding value (gEBVs) are calculated using relationships based on genome-wide markers within the DNA in place of pedigree based information

True

How important are exact phenotypes for the calculation of genomic breeding values?

Very important

The AquAdvantage Salmon, which grow to normal adult size in half the time is produced using the techniques of

Transgenesis and heat-shocking of eggs

By which 3 properties are a stem cell defined?

Ability to self-renew by asymmetrical cell division, high proliferation properties and differentiation potency

In which of these areas may stem cells be applied or utilized?

All of the above

Hematopoietic stem cells used for leukemia therapy have been shown to be truje stem clls by the following methodology

In vivo serial transplantation of bone marrow grafts

Embryonic stem cells are isolated from

The inner cell mass of an embryo

The transcription factors oct3/4, Sox2, Klf4, and C-Myc are collectively known as

The yamanaka factors of induced pluripotent stem cells

Allogenic/allogeneic cells are defined as

Cells from a different individual of the same species

Choose the most appropriate answer. Engineering of tissues relies on the interplay of

Cells, scaffolds, cytokines and probably mechanical cues

Stromal vascular fraction refers to

The nucleated cell fraction of adipose tissue

The karyotype in figure 1 is most likely of (18+x and y)

Pig

The abnormality in this karyotype (pig) is a

Robertsonian translocation

The animal in Figure 1 (pig) is expect to present with

Low sperm count

The owner of the animal in figure 1 (pig) would be advised to

Not breed/Cull

Tagging of a foreign gene to which of the following helps in visual confirmation of a transgene?

Green fluorescent protein gene

In which state will the chromatin be condensed (heterochromatinized) and DNA unreadable leading to a state of transcriptional silencef?

Histone is deacetylated and DNA Is methylated

What is the difference between prognostic, diagnostic, and surrogate biomarkers?

Analyte that provides information on likely course of disease in an untreated individual or likelihood of respose to a given therapy; analyte that helps achieve clinical diagnosis; analyte that signifies the presence of an endpoint (e.g. Death); respectively

A DNA test for copper toxicosis detection has been developed for Bedlington Terriers. The test identifies deletion of part of which gene?

COMMD1

Cancer in dogs and cats is similar to cancer in people because cancer (CHOOSE ONE)

Has a genetic basis

In animals and people, the most common cause of cancer is (CHOOSE ONE)

Environment

A 3 year old female spayed Boxer is presented to the veterinarian and you with an enlarged right popliteal lymph node. A FNA of the popliteal lymph node is examined cytologically and reveals a monomorphic population of large blastic lymphocytes. This enlarged lymph node is MOST LIKELY due to. (Choose ONE)

Neoplasia

An 8 year old female spayed domestic shorthair cat has diarrhea, occasional vomiting, and weight loss. A biopsy of the cat's intestine reveals lymphocytes, which the pathologist cannot with certainty diagnose as cancer! The astute student veterinarian suggests the lymphocytes can be sent to a laboraotry for a clonality test (also known as a polymerase chain reaction for antigen receptor rearrangements [PARR] test) If the clonality test reveals only one band then the clinicians will diagnose (choose ONE)

Lymphoma arising from a single clone of cells

A 6 year old female spayed Scottish terrier is presented to the veterinarian and you with hematuria (blood in her urine), pollakiuria (increased frequency of urination), and stranguria (straining to urinate). The dog is subsequently diagnosed with transitional cell carcinoma (TCC), which is a cancer of her lowr urinary tract. The owner notes that a group of closely related Scottish Terriers in different homes in differrent provinces is having more cancer than expected! You hypothesize the most likely mechanism for the increased incidence of cancer in this group of scottish terriers is (choose ONE)

A shared tumour suppressor gene allele mutation

A 1 year old intact male cat is presented for dyspnea (difficulty breathing) to the veterinarian and you. Together, using cytological examination of fluid from the cat's thorax, you diagnose a mss in the cat's chest as lymphosarcoma. Using an ELISA (enzyme linked immunosorbent assay) blood test, you also diagnose the cat is feline leukemia virus positive! You hypothesize the feline leukemia virus caused by lymphosarcoma by (choose the most likely mechanism)

Increased expression of an oncogene

Hypothetically, in the laboratory, a Golden Kraken's kidney cell could become a cancer cell by which ONE of the following steps?

Gaining a mutant oncogene, losing no tumour suppressor genes, and upregulating telomerase

Canine aggression is thought to be mediated by

The effects of multiple genes and interactions with environmental factors

Friendliness in ktitens has been found to be related to which of the following?

Friendliness of the sire

Which test for feline ringworm has the LOWEST risk of a FALSE positive result

Fungal culture

During embryogenesis, cell populations proliferate by

Mitosis

An embryonic eye is developing normally. What is the embryologic origin of the lens?

Surface ectoderm

What substance causes regression of the mesonephric ducts?

Regression is passive and doesn't require hormones

Skeletal muscle cells of the trunk ultimately arise from cells that were located in the

Paraxdial mesoderm

Which of the following genes is considered the master gene for eye development?

Pax-6

At what stage of the life history of many cells are cell adhesion molecules lost?

Migration

What extraembryonic membrane directly interfaces with the endometrium to form the placenta?

Chorion

The intermediate mesoderm is the precursor of which of the following?

Kidney

Where do the first blood cells of the embryo form?

Yolk sac

What tissue layer determines the nature of the hairs and other adnexal structures that form in developing skin?

Dermis

What is the principal function of the AER in the developing limb?

Stimulating outgrowth of the developing limb

If the somites close to a limb-forming region are removed, which of the following will be missing in the limb

Muscles

The cell bodies of the motor neurons of a spinal nerve arise from the

Basal plate

A cat delivers 5 cute kittens, the owners notice a few days later that one kitten has not passed any fecal material and develops abdominal swelling. Which of the following may be a condition in this little kitten that would explain the symptoms?

Both A and B are possible explanations (Anal atresia and atrasia ani)

A boston terrier is brought to a veterinary clinic for a check up after its adoption. The veterinarian palpates a right testicle within the scrotum but not one within the left scrotal sac. What is the most likely reason for this anomaly?

Both C and D are correct (Cryptorchidism of the left testicle, undescended left testicle)

Following birth, circulation from the placenta to the fetus ceases. Which of the following fetal structures act as a shunt of the placental circulation, allowing the blood to mostly bypass the pulmonary circuit?

Ductus arteriosus

Organs such as those of the digestive system are composed of several types of tissue, each derived from a different germ layer. Which of the following statements is correct regarding the digestive system origins?

Neural crest cells give rise to the myenteric plexus

Tissue recombination experiments in the developin respiratory system showed:

Tracheal endoderm combined with bronchial mesoderm results in lung budding

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