Book summary - core DNA technologies

4 important questions on Book summary - core DNA technologies

Nucleic acid hybridization assay?

  • A probe population often labbeld with fluorphore is used to interrogate the test sample.
  • With small probes you can identify alleles that differ by a single nucleotide. Base mismatch are not tolerated.
  • With long probes you can identify similarity, the hybridization can tolerate base mismatching.

Chromosome in situ hybridization?

Labeled DNA probe is hybridized against chromosomal DNA that has been denatured in situ.
  • FISH on of them.

ABI SOLiD technique?

Sequencing by oligonucleotide ligation detection.
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Ion Torrent systems

dNTP incorporating causes the release of H+ that can be detected as an eclectic signal

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