Spermatogenesis - sperm diagnostics
3 important questions on Spermatogenesis - sperm diagnostics
The predictive parameters of sperm for sperm diagnostics?
- motility
- volume and the concentration van the sperm
- the darkest yellowish colour -> higher concentration of spermatozoa
- morphology
How to calculated the concentration of sperm with the Burker counter chamber?
- You count the sperm cells in 100 squares of the Burker counter chamber.
- focus on sperm heads
- Count two borders of a square, it does not matter which sides you do not count
- 1 square is 1/4000 mm^3
- calculation for the concentration of sperm cells: 100 square spermcells X dulitionfactor X 4000/100 (we did this X 1000 to get from mm3 to ml)
- 159 x 200 x 4000/100 x 1000 -> 1272x10^6 million spermcells/ ml
How do you evaluate the morphology of the sperm?
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