Surgical procedures

5 important questions on Surgical procedures

What can be possible sources of contamination during surgery?

  • Surgery room
  • Surgeon
    • Hair, Breath, Skin
    • Sterile gloves, clean lab coat, hairnet, surgery mask
  • Instruments
  • Animal
    • Fur --> remove hair and treat skin with antiseptic
    • Faeces, Breath
  • Other material and solutions
    • instruments needs to be sterilize

What are the pre-operative preparation for surgery?

  • Checklist equipment and necessities
  • Asses animal health status
  • Pre-emptive analgesia
  • Hair removal
  • Animal positioning

What is most occurring complications in surgery?

Bleeding
  • ideal environment for bacterial growth
  • lead to poor recovery and stress
  • increase recovery time
  • increase chances of death
  • may introduce research variables


Important to think about what kind of issue you ar working with.
  • Muscle tissue is highly vasculated
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What is used for wound treatment? (instruments)

  1. Suturing
    • restore original anatomy
    • needle holder
  2. Needles
  3. Thread
    • absorbable thread is used in long term experiment, absorption start after two weeks
    • non-absorbable thread is used in short term experiments or when thread can be removed manually
      • is used to close of tissue, then you don't want absorption, it will get lose.


Interrupted stich is advised (if continuous stitch get lose, then the stitch come lose all together)

  • disadvantage: pressure may not be equal distributed
  • for secure suture, place surgeons - square - square knot
    • square knot holds more grip than slip knot

What is the post-operative care?

  1. Keep animal warm
  2. Animal does not het dehydrated (warm saline to prevent)
  3. Pain relief
  4. Make sure animals can reach their food and water
  5. Daily inspect the animal for weight loss, biting, infection, signs of discomfort

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