Microbiology - Metagenomics

16 important questions on Microbiology - Metagenomics

How does metagenomics improve traditional microbiology?

It bypasses the need for microbe cultivation by directly analizing the genetic material extracted from environmental samples.

How does metagenomics enable a holitic view?

The discovery of novel genes, enzymes and metabolic pathways.

What is shotgun metagenomics?

A method that sequences all DNA from a microbial community, capturing the full range of organisms and their functional genes.
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How can metagenomics be used in health?

To understand more about the human microbiome and its role in health and disease and hormonal syndromes.

How can metagenomics be used in the environment

To give information about biogeochemical cycles and microbial roles in cycles and can asses the health of soil, water pollution and nutrient cycling.

How can metagenomics be used in biotechnology?

For discovery of novel microbes, enzymes, antibiotics and other bioactive compounds.

How can metagenomics be used in paleogenomics?

To study ancient DNA from fossils to understand past ecological roles, environments and evolutionary relationships.

How can metagenomics be used in forensic sciences?

For the identification of microbial and animal traces/signatures for forensic investigations.

What is functional profiling?

What are organisms doing, so determining the metabolic potential and functional capabilities of individuals by analyzing the genes present in each genome.

What is ecological relationships?

Looking at why organisms are there, to understand more about the interactions between the different community members and their impact on the ecosystem.

What is crucial for accuracy of contigs and MAGs?

Manual revision and validation of the results.

What microbes are more difficult to discover in sequencing?

The ones that are less abundant in the sample, they are not much amplified and little present in the samples.

Why is metagenomics a paradigm shift?

Because it bypasses the need to culture organisms.

What is a limitation of metagenomics?

Sample complexity.

Why is taxonomy not the same as function?

Taxonomy identifies the types of organisms present in a sample and does not gain information about the functions of the organisms.

What is the difference between genomics and metagenomics?

Genomics looks at one organism and metagenomics looks at all the organisms in a sample.

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