PET - Preclinical research - In vitro
5 important questions on PET - Preclinical research - In vitro
Which three types of assays should you do in vitro?
- Binding assay
- internalization assay
- Efflux assay
You have two types of binding assays. Which ones?
- Saturation binding: when is 50% of the receptors occupied
- Competitive binding: when is 50% of the receptors occupied with the labeled tracer in presence of a cold (non labeled tracer)
How do you know what bmax is (target density) aantal bindingsplaatsen? Wat kan je nog meer zien in de grafiek?
Je kunt dan op 50% de Kd zien (dissociation constant)
Kd= off/on
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In practice, what is a difference between internalization and efflux assay?
Measure the radioactivity by gamma or beta counter and normalize to amount of protein Present data in fmol/mg
Gaat omhoog met tijd
- Efflux: collect free radio tracer.
Measure the radioactivity by gamma or beta counter and normalize to amount of protein Present data as % of internalized dose retained in cells
Gaat omlaag met tijd.
When is a signal less or high; related to internalization and efflux?
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