PET - Preclinical research - In vivo
11 important questions on PET - Preclinical research - In vivo
How can you measure the biodistribution in rats?
- Inject tracer
- Sacrifice the animals at different timepoints
- Weight the organs and count the gamma radioactivity.
- Data is analysed and presented as %ID/g
In which organs you will see high percentages in the first timeperiod?
Blood clearance.
If the compound if lipophilic more tendency to go to the liver. So it can differ per compound.
Where does tracer metabolism happen mostly?
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What kind of compound is not usefull?
Why would you use PET to see what happens with the compound? (5)
- Highly sensitive
- quantifiable (SUV)
- Dynamic imaging
- Functional information (metabolism)
- Three dimensional images
With a PET scan you can see the distribution of the compound. You have two options, which ones?
- Static => inject tracer => wait => in the pet scanner; tells you the distribution of the tracer etc. (Used when there are very slow dynamics, when there is no clinical value)
- Dynamic => inject tracer and start imaging immediately. The full imaging you fractionate into different parts. (1h, per 10 min for example). You see a timeframe of the tracer contribution
About what gives dynamic PET scan information?
What are quantification methods of PET?
- SUV in Bq/ml or cc.
- % injected dose
- comparing organ of interest with reference organ
- kinetic modeling = with blood samples. The concentration of tracer in blood plasma in an artery measured over time
What do you alway need to take into account when quantificating PET tracer uptake?
So you have to look for metabolites. Where? What can you do?
Organs => needs to be considered during image analysis
Do this:
- Radiometabolite adjusted arterial input function is crucial for the exact quantification of the PET radiotracers (especially when no appropriate reference region is available)
- Represents the concentration of the injected radiotracer in arterial blood over time.
What is PET tracer quantification: Image derived input function? Are there downsides?
Downsides:
- Not always possible, depend on the tracer distribution
- Challenging to accurately identify the left ventricle or the aorta
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