Cell models - Cell lines
7 important questions on Cell models - Cell lines
Why do we use human cells for toxicity?
- Poor prediction of adverse events
- Valid ethical concerns
- High costs and time
- Not suited for molecular investigation on cellular levels
What are cell lines, how different from primary cells?
Made by human or cancer cells that are immortal from themself.
Name some characteristics of cancer cell lines (5)
- Highly proliferative/glycolytic
- Loss of contact inhibiton => delen niet verder wanneer ze een ander raken => daarom krijg je een enkele laag.
- 50% p53 mutation
- Immortal (spontaneous or by a virus)
- Abnormal karyotype
- Higher grades + faster learning
- Never study anything twice
- 100% sure, 100% understanding
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What are limitations of cancer cells lines? (4)
➢ Lack of metabolizing enzymes => no p450 to metabolize drugs.
➢ Oncogenes tumour suppressor genes
➢ Abnormal behavior, no controlled cell numbers
What are characteristics of normal cell lines.
- Normal karyotype
- Still contact inhibition
- Immortal (expression of telomerase)
How are cell lines made immortal?
➢ In combination with viral proteins.
SV40 (large T antigen) or temperature induced (SV40ts)
What are limitations of normal cell lines? (3)
➢ Not available for all cell types
➢ Time consuming to produce
➢ Sometimes more expensive (license fees)
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
- Get the grades you hope for
- 100% sure, 100% understanding
