Mitochondrial toxicity and autophagy
10 important questions on Mitochondrial toxicity and autophagy
What is in the inner membrane space?
Cytochrome C is here.
What kind of membrane does a mitochondria have?
What can pass the outer mitochondrial membrane? And how?
Via porins.
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What can pass the inner mitochondrial membrane?
Permeable to O2, CO2, H2O. It is the site of the electron transfer. It also contains cardiolipin.
What is the role of cytochrome C?
- It is a component of the electron transport chain.
- Heam group is important => it transfect electrons by undergoing oxidation and reduction reactions.
- It transfers electrons from complex III and complex IV.
What is the role of cytochrome C in apoptosis?
What is the role of cardiolipin?
- Het is een glycerol en een glycerol zit altijd in de membranen, zo hier ook in het membraan van de mitochondria.
- Essential: Unique structure allows it to interact with various proteins in the mitochondrial membrane. Including complex IV (cyt c oxidase) and V (ATP synthase).
What is the role of cardiolipin in apoptosis?
How many ATP are generated via aerobic phosporylation (creb cycle + oxidative phosphorylation)?
(2 via glycolysis = anaerobic)
What drugs/molecules inhibit these steps?
Antimycin
Cyanide co
Oligomycin: inhibits ATP synthase
Uncoupling agent: uncouples the proton gradient.
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