Metabolomics - Different technologies - NMR
4 important questions on Metabolomics - Different technologies - NMR
What is nuclear magnetic resonance?
- Waar kom je achter: full structure of small molecules aanwezig zijn en hoeveel.
- Vergelijk met MRI-scan
- Je plaatst de moleculen in een magnetisch veld
- Je geeft een RF signal
- They move
- This move is specific -> you get a radio signal
What is a unique characteristic of NMR?
- how they move
- change conformations
- how strong they bind.
Why can't Mass Spectrometry do this?
MS looks a separate molecules in a vaccum.
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Name a kind of NMR?
Name what important is in reading the signals? (3)
- Chemical shifts for equivalent protons (often 1 molecule -> often several signals) => piek positie zegt iets over het soort H atoom, CH3 bijvoorbeeld. Of aromaten.
- Splitting patterns => how many buren. Triplet, quartet.
- Different intensities (relating to number of protons)
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