Reactive oxygen species - ROS

5 important questions on Reactive oxygen species - ROS

  • Names of ROS.  
  • Causes of ROS (intra and extra cellular)
  • What are protective reactions
=> building up to anti-oxidants.

  • H2O2. O2. OH.
  • Intra: ETC, CYP, NADPH oxidases. Extra: macrophages
  • NRF2: Nf-kB, HIF
  • Antioxidant: GSH.

Where do we find the NOX family?

In phagocyt and non phagocyt cells. => the ROS will be used as defense for wrong toxins.
In non phagocyts they are important for signal transduction.

Which of the ROS, is the most radical?

OH*.
Estimate: 10^9 ROS/human cell/hr
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What types of antioxidant defenses do we have?

Enzymatic (catalase, glutathione peroxidase) and nonezymatic (vit C en E).

What are catalyst for radical formation?

Fe+

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