Metabolomics - Possible workflows
4 important questions on Metabolomics - Possible workflows
Explain how to go from sample to spectrum?
- Start with a biological sample
- Quenching = arresting metabolism
- Extraction procedure
- Chromatography (geeft pieken weer maar dit is niet uniek)
- Mass spectrometry (geeft massa/lading verhouding, info over de moleculaire structuur, kan hele lage concentraties meten)
- Translate result to metabolites
Why quenching; stopping the metabolism before analyzing?
Je stopt de enzymactiviteit, bevriest het metabolism.
How does sample preparation work? Wat zijn verschillende manieren van extractie? (3)
- Liquid extraction = neerslaan van proteins
- Liquid-liquid extraction = polair vs apolair
- Solid phase extraction = verwijderen van zouten
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Why is sample preparation relevant?
- Sample clean-up
- Increase of sensitivity/ concentration
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