Crystallization and matrix formation - Freezing and thatwing - Antifreeze proteins
4 important questions on Crystallization and matrix formation - Freezing and thatwing - Antifreeze proteins
What are antifreeze proteins and how does it work?
What is the main difference between using cryoprotectants and antifreeze proteins?
What is the difference between using cryostabilizers and antifreeze proteins?
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When using antifreeze proteins, the driving force to crystallize is still present, there is only kinetic inhibition. What does this mean?
- If sufficient nuclei are present (lowering T too much) --> not sufficient antifreeze proteins present --> crystallisation will still happen
- Only crystal growth is inhibited. Other collapse processes, such as oxidation, or enzymatic browning may still take place, since the matrix in this case is certainly in the rubbery (WLF kinetics) zone. Hence it will be susceptible to collapse phenomena.
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