Gut brain axis, the gut side - Platforms to investigate food-gut microbiome interactions
5 important questions on Gut brain axis, the gut side - Platforms to investigate food-gut microbiome interactions
What are Next-Generation Sequencing (NGS) and Mass Spectrometry (MS), and what type of biological information does each provide in microbiome research?
What are the four main omics technologies used to study the gut microbiome, and what do they measure?
(NGS)Metagenomics; measures what microbes and microbial genes are present as well as relative abundance ((DNA)
(NGS)Transcriptomics; measures o.a. The mRNA present
(MS)Proteomics; measure proteins present
(MS)Metabolomics ; measures metabolites present
How do GC-MS and LC-MS differ in methodology, and what type of compounds/metabolites can each detect?
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What is volatilomics and how can mVOCs be used as a biomarker approach to study the microbiome?
Which metabolites are measured using GC-MS and LC-MS?
LC-MS measure the metabolites AAs, BCAAs, TRP metabolites
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